Intracellular transport of material is mainly mediated by vesicular trafficking.
This consists in the formation of a vesicle on one compartment, its transport over "long" distances, and its fusion with a second compartment.
Most of the intracellular fusion events are catalyzed by SNAREs.
Yeast vacuoles fuse in a SNARE-dependent manner.
The first step of the reaction consists in the dissociation of pre-existing SNARE complexes to allow them interacting in trans.
This is mediated by the chaperones Sec18 and Sec17.
Polyphosphate interacts with calcium.
The picture illustrates the phase separation in a mixture of polyphosphate and calcium. That this phenomen would be relevant in vivo still remains to be determined.
Vacuoles (in red) are visualized in living cells. The grey signal marks the cell wall.
How do inositol pyrophosphates influence homotypic vacuole fusion and membrane trafficking?
Deletion of KCS1 induces fragmentation of vacuoles (here shown in grey). The phenotype is rescued by expressing the mouse inositol hexakisphosphate kinase.
BY4741 kcs1∆ + mouse IP6K1
I am a postdoctoral fellow at the Department of Biochemistry (DB) at the University of Lausanne, where I am studying membrane trafficking and organelle homeostasis in the group of Andreas Mayer.
My current research interests are: